The conserved arginine required for AvrRps4 processing is also required for recognition of its N-terminal fragment in lettuce.
Identifieur interne : 000029 ( Main/Exploration ); précédent : 000028; suivant : 000030The conserved arginine required for AvrRps4 processing is also required for recognition of its N-terminal fragment in lettuce.
Auteurs : Jianbin Su [États-Unis] ; Quang-Minh Nguyen [Oman] ; Ashten Kimble [Oman] ; Sharon M. Pike [États-Unis] ; Sang Hee Kim [Corée du Sud] ; Walter Gassmann [États-Unis]Source :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2020.
Abstract
Pathogens utilize a repertoire of effectors to facilitate pathogenesis, but when the host recognizes one of them it causes effector-triggered immunity. The Pseudomonas type III effector AvrRps4 is a bipartite effector that is processed in planta into a functional 133 amino acid N-terminus (AvrRps4-N) and 88 amino acid C-terminus (AvrRps4-C). Previous studies found AvrRps4-C to be sufficient to trigger HR in turnip. In contrast, our recent work found that AvrRps4-N, but not AvrRps4-C, triggered HR in lettuce whereas both were required for resistance induction in Arabidopsis. Here, we initially compared AvrRps4 recognition by turnip and lettuce using transient expression. By serial truncation, we identified the central conserved region consisting of 37 amino acids as essential for AvrRps4-N recognition, whereas the putative type III secretion signal peptide or the C-terminal 13 amino acids were dispensable. Surprisingly, the conserved arginine at position 112 (R112) that is required for full-length AvrRps4 processing is also required for the recognition of AvrRps4-N by lettuce. Mutating R112 to hydrophobic leucine or negatively charged glutamate abolished the HR-inducing capacity of AvrRps4-N, while a positively charged lysine at this position resulted in a slow and weak HR. Together, our results suggest an AvrRps4-N recognition-specific role of R112 in lettuce.
DOI: 10.1094/MPMI-10-20-0285-R
PubMed: 33147120
Affiliations:
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Pike</name><affiliation wicri:level="2"><nlm:affiliation>University of Missouri, 14716, Columbia, Missouri, United States; pikes@missouri.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>University of Missouri, 14716, Columbia, Missouri</wicri:regionArea><placeName><region type="state">Missouri (État)</region></placeName></affiliation></author><author><name sortKey="Kim, Sang Hee" sort="Kim, Sang Hee" uniqKey="Kim S" first="Sang Hee" last="Kim">Sang Hee Kim</name><affiliation wicri:level="1"><nlm:affiliation>Gyeongsang National University, 26720, Division of Applied Life Science, Jinju, Gyeongsangnam-do, Korea (the Republic of); sangheekim@gnu.ac.kr.</nlm:affiliation><country wicri:rule="url">Corée du Sud</country><wicri:regionArea>Gyeongsang National University, 26720, Division of Applied Life Science, Jinju, Gyeongsangnam-do</wicri:regionArea><wicri:noRegion>Gyeongsangnam-do</wicri:noRegion></affiliation></author><author><name sortKey="Gassmann, Walter" sort="Gassmann, Walter" uniqKey="Gassmann W" first="Walter" last="Gassmann">Walter Gassmann</name><affiliation wicri:level="1"><nlm:affiliation>University of Missouri-Columbia, Plant Microbiology and Pathology, 371c Life Sciences Center, Columbia, Missouri, United States, 65211-7310; gassmannw@missouri.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>University of Missouri-Columbia, Plant Microbiology and Pathology, 371c Life Sciences Center, Columbia, Missouri, United States</wicri:regionArea><wicri:noRegion>United States</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pathogens utilize a repertoire of effectors to facilitate pathogenesis, but when the host recognizes one of them it causes effector-triggered immunity. The Pseudomonas type III effector AvrRps4 is a bipartite effector that is processed in planta into a functional 133 amino acid N-terminus (AvrRps4-N) and 88 amino acid C-terminus (AvrRps4-C). Previous studies found AvrRps4-C to be sufficient to trigger HR in turnip. In contrast, our recent work found that AvrRps4-N, but not AvrRps4-C, triggered HR in lettuce whereas both were required for resistance induction in Arabidopsis. Here, we initially compared AvrRps4 recognition by turnip and lettuce using transient expression. By serial truncation, we identified the central conserved region consisting of 37 amino acids as essential for AvrRps4-N recognition, whereas the putative type III secretion signal peptide or the C-terminal 13 amino acids were dispensable. Surprisingly, the conserved arginine at position 112 (R112) that is required for full-length AvrRps4 processing is also required for the recognition of AvrRps4-N by lettuce. Mutating R112 to hydrophobic leucine or negatively charged glutamate abolished the HR-inducing capacity of AvrRps4-N, while a positively charged lysine at this position resulted in a slow and weak HR. Together, our results suggest an AvrRps4-N recognition-specific role of R112 in lettuce.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33147120</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><PubDate><Year>2020</Year><Month>Nov</Month><Day>04</Day></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>The conserved arginine required for AvrRps4 processing is also required for recognition of its N-terminal fragment in lettuce.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-10-20-0285-R</ELocationID><Abstract><AbstractText>Pathogens utilize a repertoire of effectors to facilitate pathogenesis, but when the host recognizes one of them it causes effector-triggered immunity. The Pseudomonas type III effector AvrRps4 is a bipartite effector that is processed in planta into a functional 133 amino acid N-terminus (AvrRps4-N) and 88 amino acid C-terminus (AvrRps4-C). Previous studies found AvrRps4-C to be sufficient to trigger HR in turnip. In contrast, our recent work found that AvrRps4-N, but not AvrRps4-C, triggered HR in lettuce whereas both were required for resistance induction in Arabidopsis. Here, we initially compared AvrRps4 recognition by turnip and lettuce using transient expression. By serial truncation, we identified the central conserved region consisting of 37 amino acids as essential for AvrRps4-N recognition, whereas the putative type III secretion signal peptide or the C-terminal 13 amino acids were dispensable. Surprisingly, the conserved arginine at position 112 (R112) that is required for full-length AvrRps4 processing is also required for the recognition of AvrRps4-N by lettuce. Mutating R112 to hydrophobic leucine or negatively charged glutamate abolished the HR-inducing capacity of AvrRps4-N, while a positively charged lysine at this position resulted in a slow and weak HR. Together, our results suggest an AvrRps4-N recognition-specific role of R112 in lettuce.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Su</LastName><ForeName>Jianbin</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>University of Missouri Columbia, 14716, 314 LSC, Columbia, Missouri, United States, 65211; suj@missouri.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Quang-Minh</ForeName><Initials>QM</Initials><AffiliationInfo><Affiliation>Gyeongsang National University, 26720, Division of Applied Life Science, Jinju, Gyeongsangnam-do, Korea (the Republic of); nguyenquangminh4595@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kimble</LastName><ForeName>Ashten</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>University of Missouri, 14716, Columbia, Missouri, United States; ashten.kimble.ext@bayer.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pike</LastName><ForeName>Sharon M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>University of Missouri, 14716, Columbia, Missouri, United States; pikes@missouri.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Sang Hee</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Gyeongsang National University, 26720, Division of Applied Life Science, Jinju, Gyeongsangnam-do, Korea (the Republic of); sangheekim@gnu.ac.kr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gassmann</LastName><ForeName>Walter</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>University of Missouri-Columbia, Plant Microbiology and Pathology, 371c Life Sciences Center, Columbia, Missouri, United States, 65211-7310; gassmannw@missouri.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>4</Day><Hour>17</Hour><Minute>10</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33147120</ArticleId><ArticleId IdType="doi">10.1094/MPMI-10-20-0285-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li><li>Oman</li><li>États-Unis</li></country><region><li>Missouri (État)</li></region></list><tree><country name="États-Unis"><noRegion><name sortKey="Su, Jianbin" sort="Su, Jianbin" uniqKey="Su J" first="Jianbin" last="Su">Jianbin Su</name></noRegion><name sortKey="Gassmann, Walter" sort="Gassmann, Walter" uniqKey="Gassmann W" first="Walter" last="Gassmann">Walter Gassmann</name><name sortKey="Pike, Sharon M" sort="Pike, Sharon M" uniqKey="Pike S" first="Sharon M" last="Pike">Sharon M. Pike</name></country><country name="Oman"><noRegion><name sortKey="Nguyen, Quang Minh" sort="Nguyen, Quang Minh" uniqKey="Nguyen Q" first="Quang-Minh" last="Nguyen">Quang-Minh Nguyen</name></noRegion><name sortKey="Kimble, Ashten" sort="Kimble, Ashten" uniqKey="Kimble A" first="Ashten" last="Kimble">Ashten Kimble</name></country><country name="Corée du Sud"><noRegion><name sortKey="Kim, Sang Hee" sort="Kim, Sang Hee" uniqKey="Kim S" first="Sang Hee" last="Kim">Sang Hee Kim</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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